SPE Sorbent

SPE Sorbents

Silica-based sorbents

Flash grade

Granular, irregular-shaped particle

40-63 µm particle diameter range (40-75 is ok)

Surface area = 500-600 square meters/gram

Pore size = 60Å

• C2       Reverse phase, ethyl (C₂), non-endcapped, 5-10% carbon loading
• C4       Reverse phase, butyl (C₄), non-endcapped, 5-10% carbon loading
• C8       Reverse phase, octyl (C₈), endcapped and non-endcapped,

8-10% carbon loading

• C18     Reverse phase, octadecyl (C₁₈), endcapped and non-endcapped,

15-22% carbon loading

• Cyclohexyl    Reverse phase (C₆H₁₁)
• Phenyl           Reverse phase (C₆H₁₁)
• SAX    Strong anion-exchange, quaternary amine (N⁺)
• WAX   Weak anion-exchange, Amino (NH₂)
• SCX    Strong cation-exchange, aromatic benzene sulfonic acid (C₆H₅SOH)
• WCX   Weak cation-exchange, Carboxylic acid (COOH)
• CN      Cyano (CN)
• Diol     (COHCOH)
• Silica  Granular flash grade (SiOH)

Particle diameter = 40-75 µm

Pore diameters = 70Å, 100Å and 300Åneed all three.

• Florisil (Mg₂SiO₃) (150-200 µm particle diameter)

Alumina silica-based sorbents (non-silica)

Larger diameter particle (50-200 µm) or (60-150 µm)

Surface area = 150-200 square meters/gram

• AL-B (basic)
• AL-N (neutral, Al₂O₃)
• AL-A (acidic)

Cellulose-based

• BNDC                        Benzoylated napthoylated DEAE cellulose

Gillam et al., Biochemistry, 6, 3043 (1967).

Polymer-based

• Hydrophilic Divinylbenzene (DVB)
• Hydrophobic Divinylbenzene (DVB)

Agarose

• Molecular Biology Grade (low electroendosmosis) EEO=0.05-0.13
• Low Melting Temperature, EEO<0.12

Biological Buffers

• Tris Buffer: Tris( Hydroxymethyl)Aminomethane (mw 121.1)
• Tricine Buffer: N(Hydroxymethyl)Methylglycine (mw 179.2)
• Hepes (sodium salt) Buffer: N(2-Hydroxymethyl)Piperazine-N-[2-Ethanesulfonic acid] (mw 260.3)
• MOPS (sodium salt) Buffer: 3-[N-Morphinolino]Propane-Sulfonic Acid (mw 231.3)