Antibiotic residue detection Chloramphenicol(CAP) ELISA testing

      Chloramphenicol(CAP) ELISA rapid Test Kit 

1.Principle
This test kit is based on the competitive enzyme immunoassay for the detection of
Chloramphenicol in the sample. The coupling antigen is pre-coated on the micro-well stripes. The
Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes
compete for the anti-Chloramphenicol antibody. After the addition of the enzyme conjugate, the
TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative
correlation with the Chloramphenicol in it. This value is compared to the standard curve and the
Chloramphenicol concentration is subsequently obtained.
2.Technical specifications

Sensit: 0.05 ppb

Detection limit
Intestine..................................................................................................0.1 ppb
Honey ................................................................................................... 0.15 ppb
Milk....................................................................................................... 0.05 ppb
Urine and serum.....................................................................................0.1 ppb
Chicken/liver, pork/liver, fish, shrimp....................................................0.05 ppb
Recovery rate
Chicken/liver, pork/liver, fish, shrimp..................................................80%±10%
Honey .................................................................................................70%±10%
Milk.....................................................................................................85%±15%
Cross-reaction rate
Chloramphenicol ....................................................................................... 100%
Thiamphenicol .........................................................................................< 0.1%
Florfeniol................................................................................................. < 0.1%
3.Components
1) Micro-well strips: 12 strips with 8 removable wells each
2) 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3) Enzyme conjugate (12 mL)...................................................................red cap
4) Antibody concentrated solution (1 mL)................................................blue cap
5) Substrate A solution (7 mL)............................................................... white cap
6) Substrate B solution (7 mL)............................................................... black cap
7) Stop solution (7 mL).........................................................................yellow cap
8) 20× concentrated washing buffer (40 mL).........................................white cap
9) 2× concentrated redissolving solution (50 mL)........................transparent cap 

4.Storage and expiry date
Storage: store at 2 to 8 °C, not frozen.
Expiry date: 12 months; date of production is on box.

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We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.

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