catalyzes the breakdown of hydrogen peroxide, a toxic substance to organisms, to water and molecular oxygen
Factors Affecting the Activity of Catalase
Catalase and its rate of activity are affected by many factors, including temperature, pH, salt concentration, amount of substrate and the presence of inhibitors or activators.
(1)Temperature, it is an important factor in all biochemical reactions, as high temperatures cause denaturation of enzymes. As the enzyme denatures, it changes the conformation, causing the substrate to bind less efficiently, therefore decreasing the reaction. However, until that maximum temperature (which is different for each type of catalase) is reached, the reaction will increase as the temperature increases.
(2)The pH, its is the measure of acidity or hydrogen ion concentration with a solution, is measured on a scale of 0-14. As a solution becomes more acidic (below 7), the enzyme can gain a hydrogen ion from the solution and as the solution becomes more basic (above 7), it can lose a hydrogen ion. Either of these extremes can reduce the rate of the reaction as it changes the chemical bonds of the catalase.
(3) Inhibitors There are two types of inhibitors: non-competitive inhibitors, which bind somewhere other than the active site, and competitive inhibitors, which bind to the active site of the catalase. Copper sulfate is a known non-competitive inhibitor of catalase, and cyanide is a known inhibitor.
Other Uses of Catalase
Because catalase is present in just about all living organisms, it is one of the most studied enzymes. This has led to scientists finding other functions for this efficient enzyme.
1. Catalase is used to treat the inside of food wrappers. The catalase prevents oxidation and therefore helps preserve the food.
2. Catalase is also used during the process of cold sterilization, which is a process to preserve milk and cheese by treatment with hydrogen peroxide.
3. The catalase is used to remove any remaining traces of the hydrogen peroxide.
Catalase is a yellowish to brown powder or liquid.
We can provide the maximum enzyme activity to 30,000,000 u/g.
0-4 °C cryopreservation to avoid direct sunlight
1kg/ Vacuum Aluminum foil bag for powder, 30kg/drum for liquid. We also can be changed according to customer’s requirement