Quicking Chloramphenicol Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Chloramphenicol residue in honey sample.
Cut-off: 0.3 ppb
Assay Time: 5 - 8 min
2. PRINCIPLE OF THE ASSAY
Quicking Chloramphenicol Rapid Test is based on competitive lateral flow immunochromatographic assay. The Chloramphenicol conjugate in the test zone will capture the immuno-gold (colloid gold- Chloramphenicol antibody conjugate), when there is very little dissociative Chloramphenicol in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Chloramphenicol is present in concentration of 0.1 ppb and above which explains a positive result.
3. KIT COMPONENT
- 10×foil pouches each containing one cassette and a desiccant
- 1×plastic canister contained 10 microwells and a desiccant
- Products Manual
4. TEST PROCEDURE
- Collect 4 g of honey sample into a 15 mL tube.
- Add 2 mL of distilled water and mix sufficiently. If there is crystal, thaw the sample in a water bath (60-80°C) beforehand.
- Add 8 mL of acetic ether into the tube. Shake for 10 min and then stand for layer.
- Suck 4 mL of the clean supernatant of upper layer into a 5 mL tube.
- Dry the liquid by blowing wind and redissolve the residue with 0.3 mL of assay buffer.
- Take out the microwells strip from the plastic canister. Take one well and tear off the film.
- Suck 0.2 mL of the extract into the well with another pipette (the reticle level is 0.2 mL content). Repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for 1 min.
- Take out the cassette from the foil pouch and place it horizontally.
- Suck the mixture in the well and gradually drop 3 drops into the assay sample hole “S”.
- Interpret the result in 5-8 min. Result after 8 min is considered as invalid.
5. INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone C indicates a positive result. If a vague T band can be seen but apparently weaker than C band, we also consider it as a positive result. Positive shows that the concentration of Chloramphenicol is above 0.1 ppb (ng/mL) in the sample.
Negative: The presence of both clear bands in C zone and T zone. (T band is close to or stronger than C band.)
Invalid: No colored band appears in C zone.
The results are negative when the test card is applied to detecting 100 ppm (μg/mL) of Tetracycline, Aminoglycosides, Beta-lactams, Sulfonamides, and Macrolides.
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not reuse the test kit.
- Do not use the test beyond its expiration date marked on the foil pouch.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
Quicking Chloramphenicol Rapid Test is a useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Chloramphenicol above 0.3ppb in honey samples. If you want a quantitative result, please adopt other method such as ELISA/ HPLC in practice.