The hCG Rapid Test Midstream is a rapid, self-performing, qualitative immunoassay for the determination of human chorionic gonadotropin (hCG) in urine specimen at 20 mIU/ml or above.
Principle of HCG Test Midstream
The hCG Rapid Test Midstream is a qualitative, solid phase, two-site sandwich immunoassay (5-6) for the detection of human chorionic gonadotropin (hCG) in urine. This test utilizes the principle of immuno-chromatography, a unique two-site immunoassay on a membrane. As the test sample flows through the membrane assembly within the test device, the colored anti-hCG-colloidal gold conjugate binds with the hCG in the sample. This complex moves further on the membrane to the test region where it is immobilized by the anti-hCG coated on the membrane leading to formation of a pink colored line which confirms a positive test result. Absence of this colored line in the test region indicates a negative test result.
The unreacted conjugate and unbound complex form a pink line, if any move further on the membrane and are subsequently immobilized by the anti-mouse antibodies coated on the membrane at the control region. This control line serves to validate the test results.
Procedure and Result Reading of HCG Test Midstream
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