L-5 methyl tetrahydrofolate calcium

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Quick Details

CAS No.:
151533-22-1
Other Names:
L-5-Methyltetrahydrofolic acid, calcium saltL-Methyltetrahydrofolate
MF:
C20H23CaN7O6(no water)
Type:
Anti-Allergic Agents
Grade Standard:
Medicine Grade

Packaging & Delivery

Packaging Detail: 25kg/drum,10g/bag
Delivery Detail: within 3-5 workdays after payment

Specifications

1.In very competitive price,
2.Quality guaranteed,
3.Fast Delivery

TESTS

PURITY TESTS

Other folates and related substances

Using a L-5-MTHF-Ca reference standard, Quantitate other folates and related substances by HPLC. The suitability of the applied HPLC system is checked daily by a "system suitability test" (see below).

 

Reference standard solution: Accurately weigh 50 mg of L-5-MTHF-Ca (L-5- methyltetrahydrofolic acid, calcium salt (Merck Eprova AG, CH-8200

Schaffhausen, Switzerland) into a 100-ml volumetric flask. Dissolve in a small quantity of water and dilute to volume.

 

Sample solution: Prepare as for the reference standard using 50 mg of the sample. 

Mobile phase solutions

A: Dissolve 7.80 g of NaH2PO· 2H2O (0.05 mol) in 1000 ml of water and adjust the pH to 6.5 with 32% NaOH. Filter and degas the solution.

B: Dissolve 5.07 g of NaH2PO· 2H2O (0.03 mol) in 650 ml of water and 350 ml of methanol (chromatography grade) and adjust the pH to 8.0 with 32%

  

NaOH. Filter and degas the solution.

 

Chromatography Conditions

Column: Hypersil-ODS, 5 µm; 250 x 4 mm (Thermo Hypersil Keystone or equivalent)

Flow rate: 1.1 ml/min

Gradient:

Time

(min)

% Mobile phase

A

% Mobile phase

B

Remark

0

100

0

Start

0 - 14

100 – 45

– 55

Linear gradient

14 – 17

45 – 0

100

Linear gradient

17 – 22

0

100

Hold

22 – 31

100

0

Reconditionin g

Temperature: Room temperature

Injection volume: 10 µl Detection: UV (280 nm) Run time: 22 min

 

Retention times given below are approximate:

Folates and related substances

Retention time (min)

4-Aminobenzoylglutamic acid (ABGA)

3.1

4α-Hydroxy-5-methyltetrahydrofolic acid

(HOMeTHFA)

4.3

D-Pyrazino-s-triazine derivative (D-Mefox)

6.1

L-Pyrazino-s-triazine derivative (L-Mefox)

6.3

Tetrahydrofolic acid (THFA)

8.5

7,8-Dihydrofolic acid (DHFA)

11.2

Folic acid (FA)

11.4

5,10-Methylenetetrahydrofolic acid (CH2THFA)

11.7

5-Methyltetrahydrofolic acid (5-MTHF)

13.6

5-Methyltetrahydropteroic acid (MeTHPA)

15.1

N2-Methylamino-5-methyltetrahydrofolic acid

(DiMeTHFA)

17.6

 

Sample analysis: Inject the reference standard solution and the sample solutions immediately after preparation, using the conditions described above.

 

(Note: After analysis, flush the column with methanol/water 85:15 and store it under the same conditions.)

 

Calculate the content of each folate (other than 5-MTHF) and related substance, X(%), listed in the above table according to the following formula:

 

X(%) = A×W× × (RF)i/A× W

where

Ai   = the peak area for each folate (other than 5-MTHF) and related substance

AS  = the peak area for the L-5-MTHF-Ca Standard

WS  = the weight (mg) of L-5-MTHF-Ca Standard

W  = the weight (mg) of the sample

S= the percent of L-5-MTHF in the L-5-MTHF-Ca Standard, calculated as

 

free acid

(RF)= Response Factor for the i-th substance (absorbance at 280 nm in the applied eluent system relative to that of L-5-MTHF)

 

Other folates and related substances

RF

ABGA

0.93

HOMeTHFA

1.11

L-Mefox and D-Mefox

1.11

DHFA

0.98

FA

0.86

MeTHPA

0.68

THFA

1.00

CH2THFA

1.00

DiMeTHFA

1.00

 

If there are any unidentified impurities, apply a RF of 1.00.

 

Calculate the total amount of "Other folates and related substances" by summing the Xfor all impurities.

 

System suitability test procedure

 

Mixed folates solution: Weigh 25 mg each of ABGA, HOMeTHFA, L-Mefox, DHFA, FA and MeTHPA (all available from Merck Eprova AG) into a 100-ml volumetric flask. Add a small quantity of water to dissolve the mixture; add some sodium hydrogen carbonate and sodium carbonate to aid the dissolution, and fill to the mark with water.

 

System suitability test solution (SST solution): Weigh accurately 50 mg of a L-

5-MTHF-Ca sample containing DiMeTHFA into a 100-ml volumetric flask. (Available from Merck Eprova AG). Add 1 ml of the Mixed folates solution and a small quantity of water to dissolve, mix and dilute to volume with water.

 

System suitability test: Inject 10 µl of the SST solution immediately. The resolution between L-5-MTHF and MeTHPA must be at least 5.

 

D-5-MethylfolateD-5-Methylfolate is quantitated by HPLC using a chromatographic system which allows separation of the D- from the L-stereoisomer. The suitability of the

applied HPLC system is checked daily by a "system suitability test" (see below).

 

Sample preparation: Accurately weigh 50 mg of the sample into a 100 ml volumetric flask. Dissolve in water and dilute to volume with water.

 

Mobile phase: Mix 970 ml of 0.03 M NaH2PO(obtained by dissolving 4.68 g of NaH2PO· 2H2O in water and diluting with water to 1000 ml) with 30 ml of acetonitrile (chromatography grade) and adjust the pH to 6.8 with 32% NaOH. Filter and degas the solution.

 

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Changsha Huir Biological-Tech Co., Ltd. (CSHR) has been dedicated to the research and development of herbal and nutraceutical ingredients since 1997. In the past 14 years, we have strictly controlled the quality from raw materials to finished products, and have made our spirits of pursuing perfect embody in our herbal extract production field.

CSHR is a professional manufacturer and developer of high-quality and standard botanical extracts, which come from nature and are good for human ... View detail

Basic Information

Product/Service (We Sell): Huperzine-A,Resveratrol,Red Clover P.E,Ginkgo Biloba Extract,Bilberry Extract  
Number of Employees: 51 - 100 People  

Trade & Market

Total Annual Sales Volume: US$10 Million - US$50 Million  
Export Percentage: 61% - 70%  

Factory Information

Factory Size (Sq.meters): 30,000-50,000 square meters  
Factory Location: Changsha. China  
Number of Production Lines: 10  
Number of R&D Staff: 5 - 10 People  
Number of QC Staff: 21 - 30 People  
Contract Manufacturing: OEM Service Offered   Design Service Offered   Buyer Label Offered  

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China (Mainland) | Manufacturer, Trading Company | Contact Details
Experience: Established 2006

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Ms. Lily Liu
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