Application: it is an important PH stable reagent in biotic experiments.
Appropriate PH is obtained by mixing mild acid and its conjugate base.
Many biological responses happen in neutral condition (usually PH 6-8),
so the buffering range is required to be in PH 6-8. In addition, the form
of acid-base of buffer should not chelate with some metallic ions.
Characteristics of biological buffer:
1, Strong water-solubility;
2. Compatibility of the acid-base dissociation balance;
3. Low membrance penetration rate;
4. Low metal chelating ability;
5. High chemical stability;
6. Low absorption in ultraviolet and visible region.