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A general purposes medium
Typical formula (g/l)
Beef Extract 3.0
Sodium Chloride 8.0
Final pH 7.3 +/- 0.2
Suspend 28g of Nutrient Agar in 1000ml of cold distilled water. Heat to dissolve and sterilise by autoclaving at 121°C for 15 minutes.
Nutrient Agar is used for the cultivation of non-fastidious microorganisms. The Beef Extract and Peptone supply a quantity of carbon, nitrogen and vitamins sufficient for the growth of most non-fastidious microorganisms (enteric bacteria, staphylococci). The APHA recommends the use of Nutrient Agar in the microbiological examination of water and dairy products. These media can be used as bases to which different materials can be added such as; carbohydrates, salts, dyes, etc., in order to obtain a selective, differential and enriched media.
Nutrient Agar was the first media utilised in microbiology and can still be routinely used in the examination of water and foods for preparing stock cultures; for the preliminary cultivation of a sample undergoing successive bacteriological examinations; and for the isolation of microorganisms from a pure culture.
Quality assurance (24 h -37°C)
S.aureus ATCC 25923: growth
E.coli ATCC 25922: growth
Dehydrated media: 15-30°C
Prepared plates: 1 month at 2-8°C
Prepared tubes and flasks: 3 months at 2-8°C
APHA (1960) - Standard Methods for Examination of Water and Wastewater.
APHA (1976) - Compendium of Methods for Microbiological Examination of Foods.