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Quant One Step qRT-PCR (Probe) Kit

FOB Price: Get Latest Price
Min.Order Quantity: 5 Set/Sets
Supply Ability: 500000 Unit/Units per Week
Port: Beijing
Payment Terms: L/C,D/A,D/P,T/T
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Quick Details

Classification:
Other
Brand Name:
TIANGEN
Model Number:
FP304
Place of Origin:
Beijing China (Mainland)

Specifications

High specificity and sensitivity, achieved by
using Quant RTase and Hotmaster Taq polymerase

  • Ordering Information:
 

Cat. no.

Quantity

FP304-01

50 preps × 50 µl

  
  • Contents and Storage:

 

Kit Components

50 preps × 50 µl

2×Quant One Step Probe qRT-PCR Master Mix*

1.3 ml

HotMaster Taq Polymerase (2.5 U/µl)

130 µl

Quant Reverse Transcriptase (for one step)

40 µl

RNase-free ddH2O

1.8 ml

* Contains dNTP Mixture, One step Probe qRT-PCR buffer and ROX Reference Dye

Store at -20°C and protected from light.

  

  • Description:

 

Quant One Step qRT-PCR (Probe) Kit provides rapid real-time quantification of RNA targets in an easy-to-handle format. The kit is compatible with TaqMan®, Molecular Beacon, etc. The kit allows both reverse transcription and gene amplification to take place in a single tube. The reaction system is highly sensitive by real-time detection of the PCR products and does not need electrophoresis analysis after PCR, which is suitable for detecting minimal amount of RNA.

 

High specificity and sensitivity in qRT-PCR are achieved by the use of Quant RTase reverse transcriptase and Hotmaster Taq DNA polymerase. Quant RTase expressed by engineering bacteria is efficient reverse transcriptase with a high affinity for RNA, which facilitates transcription through of RNA templates with high GC content or complex secondary structures. HotMaster inhibitor blocks the substrate binding site of DNA polymerases in a temperature dependent manner. Inactive polymerase-inhibitor complexes are formed at temperatures below 40°C. As the temperature is elevated to the primer-specific annealing temperature, the binding equilibrium is shifted towards complex formation only with template-specific primer, which ensures non-specific amplified products are minimized in the whole of PCR.

 

         

 

 

 

 

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Tiangen Biotech (Beijing) Co., Ltd. integrates development, manufacture, distribution and customer service in the life-science field. Our mission is to supply the customized biological reagents and professional technical services.

On the basis of abundant capital, advanced management experience, leading technology and quality controlling systems, we guarantee to provide a series of products with stable performance and high-quality for research elite worldwide.

Our products consist of ... View detail

Basic Information

Product/Service (We Sell): Nucleic Acid Purification,PCR, RT-PCR & Real-Time PCR Reagent,Cloning System,Molecular Weight Marker,Protein Product Line  
Number of Employees: 101 - 200 People  

Trade & Market

Total Annual Sales Volume: US$10 Million - US$50 Million  
Export Percentage: 11% - 20%  

Factory Information

Factory Size (Sq.meters): 1,000-3,000 square meters  
Factory Location: Building C7-3, Zhongguancun DongshengScience Park, No.66 XixiaokouRoad Haidian District, Beijing. China  
Number of Production Lines: Above 10  
Number of R&D Staff: 21 - 30 People  
Number of QC Staff: 11 - 20 People  
Management Certification: ISO9001  
Contract Manufacturing: OEM Service Offered   Buyer Label Offered  

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China (Mainland) | Manufacturer, Trading Company | Contact Details
Experience: Established 2005

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Ms. wenqian yan
Ms. wenqian yan:
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