|Classification:||Other||Brand Name:||TIANGEN||Model Number:||FP304|
|Place of Origin:||Beijing China (Mainland)|
High specificity and sensitivity, achieved by
using Quant RTase and Hotmaster Taq polymerase
50 preps × 50 µl
50 preps × 50 µl
2×Quant One Step Probe qRT-PCR Master Mix*
HotMaster Taq Polymerase (2.5 U/µl)
Quant Reverse Transcriptase (for one step)
* Contains dNTP Mixture, One step Probe qRT-PCR buffer and ROX Reference Dye
Store at -20°C and protected from light.
Quant One Step qRT-PCR (Probe) Kit provides rapid real-time quantification of RNA targets in an easy-to-handle format. The kit is compatible with TaqMan®, Molecular Beacon, etc. The kit allows both reverse transcription and gene amplification to take place in a single tube. The reaction system is highly sensitive by real-time detection of the PCR products and does not need electrophoresis analysis after PCR, which is suitable for detecting minimal amount of RNA.
High specificity and sensitivity in qRT-PCR are achieved by the use of Quant RTase reverse transcriptase and Hotmaster Taq DNA polymerase. Quant RTase expressed by engineering bacteria is efficient reverse transcriptase with a high affinity for RNA, which facilitates transcription through of RNA templates with high GC content or complex secondary structures. HotMaster inhibitor blocks the substrate binding site of DNA polymerases in a temperature dependent manner. Inactive polymerase-inhibitor complexes are formed at temperatures below 40°C. As the temperature is elevated to the primer-specific annealing temperature, the binding equilibrium is shifted towards complex formation only with template-specific primer, which ensures non-specific amplified products are minimized in the whole of PCR.