Nisin is a polypeptide produced by Lactococcus Lactis and can be digested into amino acids by a-chymotrypsinin in human intestine and stomach. The use of nisin will not affect the normal bacterial chiasmatic resistance as it happened. When the antibiotics used. It possesses anti-microbial activity against a wide range of Gram-positive bacteria , particular those that produce spores , which are the major food spoilage organisms , such as Clostridium botulinum Staphylococcus aurous Streptococcus hemolyticuslisteria monocytogenesBacillus stearothermophilusBacillus subilis etc .