1kg/bag, 25Kg /Drum or According to your requirements.
1 - 100
To be negotiated
Food Additives High Activity Lactase
We have various kinds of lactase like 10,000ALU/G,40,000ALU/G,60,000ALU/G,100,000ALU/G,AND 160,000ALU/G.
Lactase, also known as beta-galactosidase hydrolysis of lactose to produce glucose and galactose. Lactase also has the transfer function of galactosidase, galactose to lactose generated galactooligosaccharide, as a bacteria-acting factors for the development of functional foods. Biological enzyme lactase is a non-toxic side effects, by the authority of the FDA, the FCC, the WHO / FAO and JACFA assessment to determine the safety of substances.
Natural lactase usually present in the human gut. Were very low, however, when people ate dairy, because most of the human body, a lack of lactase, so difficult for them to digest and absorb the milk sugar lactose, the lactose in milk can not be decomposed use, intestinal bacteria break down the fermentation, producing a large carbon dioxide gas, resulting in intestinal expansion and peristalsis contraction to strengthen, resulting in bowel, diarrhea, bloating and other symptoms of this disease is called lactose intolerance. Lactase deficiency worldwide is widespread, especially in Asia, Africa accounted for a large proportion of our country is also a common phenomenon.
Product Name: Lactase
Other Name: Beta-Galactosidase
Strain: Aspergillus oryzae Mfg.Date: 2019.04.04
Batch Number: SA1190404 Exp.Date: 2021.04.03
Package: 25 kg/drum Quantity: 311.47 kg
PHYSICAL & CHEMICAL ANALYSIS
Odour & Taste
White to light brown powder
Slight odor of fermatation
Positive for Lactase Activity
≥ 100,000 ALU/g
Total bacteria count
E.Coli (in 25g)
Salmonella (in 25g)
Mold & Yeast count
≤ 1000 CFU/g
≤ 30 CFU/g
≤ 50 CFU/g
FDA BAM Online Ch.3
FDA BAM Online Ch.4
FDA BAM Online Ch.4
FDA BAM Online Ch.5
FDA BAM Online Ch.18
≤ 3 ppm
≤ 1 ppm
Storage: Store in cool and dry place protected from light, keep drum close when not in use.
Conclusion: Conforms to NF11 Standards.
Shelf Life:Shelf life under prescribed storage conditions and air-tight packing will be 2 Years.
The product COMPLIES with the above said specifications.
1. Improve metabolic energy utilization rate of meal like raw material soybean meal, cottonseed meal, rapeseed meal, reduce chyme viscosity, improve animal production performance and increase feed/gain ratio.
2. Completely degrade oligosaccharide of meal like raw materials, reduce nutrients residue in chyme, and increase nutrient utilization rate.
3. Eliminate flatulence, diarrhea of mono-gastric animals, deal to digestive disorders caused by oligosaccharide.
4. Ease or avoid alimentary apparatus compensatory hyperplasia, reduce animal maintenance requirements, improve feed energy valence.
For a long time, lactase production for hydrolysis of milk and milk caramel. It can also be used for the processing of a range of other dairy products such as yogurt, ice cream, desserts, butter and cheese.
We specialize in the manufacture of lactase can be used as aid digestion agent used in dietary supplements. The role of the enzyme has a transglycosylation, can also be used for the production of functional galactooligosaccharides.
When people consumed the dairy products or lactose-containing food, as a lack of lactase in the body, the lactose is digested and fermented by intestinal bacteria and forms a mass of carbon dioxide gas. It can lead to the expansiodn of intestinal tract and the strengthening of intestinal peristalsis. It brings borborygmus, diarrhea, abdominal distension an so on. It is called lactose intolerance.
The Lactase from Aspergillus oryzae has a high activity at low pH(pH3.5~5.5) making it particularly suitable for digest lactose in the stomach, and it’s therefore ideal for use in the dietary supplements as digestive aid. The dose of fungal lactase depends on the amount of the lactose and the size of meal consumed, one general guideline is 2,500 ALU per 10~13 grams of lactose consumed.We recommend a dose of 4,000 ~6,000 ALU with each lactose-containing meal. For very sensitive lactose-intolerant consumers (need a 100% conversion), 10,000 ALU per meal is recommended. Other application: Apply to process free-lactose yoghurt.
LACTASE (ACID) (β-GALACTOSIDASE) ACTIVITY
Reagents and Solutions 2.0 N Acetic Acid Dilute 57.5 mL of glacial acetic acid to 500 mL with water. Mix well, and store in a refrigerator.4.0 N Sodium Hydroxide Dissolve 40.0 g of sodium hydroxide in sufficient water to make 250 mL.Acetate Buffer Combine 50 mL of 2.0 N Acetic Acid and 11.3 mL of 4.0 N Sodium Hydroxide in a 1000-mL volumetric flask, and dilute to volume with water. Verify that the pH is 4.50 0.05, using a pH meter, and adjust, if necessary, with 2.0 N Acetic Acid or 4.0 N Sodium Hydroxide. 2.0 mM o-Nitrophenol Stock Transfer 139.0 mg of o-nitrophenol to a 500-mL volumetric flask, dissolve in 10 mL of USP alcohol (95% ethanol) by swirling, and dilute to volume with 1% sodium carbonate.o-Nitrophenol Standards 0.10 mM Standard Solution Pipet 5.0 mL of the 2.0 mM o-Nitrophenol Stock solution into a 100-mL volumetric flask,and dilute to volume with 1% sodium carbonate solution. 0.14 mM Standard Solution Pipet 7.0 mL of the 2.0 mM o-Nitrophenol Stock solution into a 100-mL volumetric flask,and dilute to volume with 1% sodium carbonate solution. 0.18 mM Standard Solution Pipet 9.0 mL of the 2.0 mM o-Nitrophenol Stock solution into a 100-mL volumetric flask,and dilute to volume with 1% sodium carbonate solution. Substrate Transfer 370.0 mg of o-nitrophenyl-β-D-galactopyranoside to a 100-mL volumetric flask, and add 50mL of Acetate Buffer. Swirl to dissolve, and dilute to volumewith Acetate Buffer. [NOTE—Perform the assay procedure within 2 h of Substrate preparation.]
Test Preparation Prepare a solution from the test sample preparation such that 1 mL of the final dilution will contain between 0.15 and 0.65 lactase unit. Weigh, and quantitatively transfer the enzyme to a volumetric flask of appropriate size. Dissolve the enzyme in water, swirling gently, and dilute with water if necessary. [NOTE—Perform the assay procedure within 2 h of dissolution of the Test Preparation.]
System Suitability Determine the absorbance of the three o-Nitrophenol Standards at 420 nm in a 1-cm cell, using a suitable spectrophotometer. Use water to zero the instrument. Calculate the millimolar extinction, M, for each of the o-Nitrophenol Standards (0.10, 0.14, and 0.18 mM) by the equation ε= An/C in which An is the absorbance of each o-Nitrophenol Standard at 420 nm and C is the corresponding concentration of o-nitrophenol in the standard. M for each standard should be approximately 4.60/mM. Perform a linear regression analysis of the absorbance readings of the three o-Nitrophenol Standards versus the onitrophenol concentration in each (0.10, 0.14, and 0.18 mM). The r 2 should not be less than 0.99.Determine the mean M of the three o-Nitrophenol Standards for use in the calculations below.Procedure For each sample or blank, pipet 2.0 mL of the Substrate solution into a 25- 150-mm test tube, and equilibrate in a water bath maintained at 37.0° 0.1° for approximately 10 min. At zero time, rapidly pipet 0.5 mL of the Test Preparation (or 0.5 mL of water as a blank) into the equilibrated substrate, mix by brief (1 s) vortex, and immediately return the tubes to the water bath. After exactly 15 min of incubation,rapidly add 2.5 mL of 10% sodium carbonate solution, and vortex the tube to stop the enzyme reaction. Dilute the samples and blanks to 25.0 mL by adding 20.0 mL of water, and thoroughly mix. Determine the absorbance of the diluted samples and blanks at 420 nm in a 1-cm cell, using a suitable spectrophotometer. Use water to zero the instrument. Calculation One lactase unit (ALU) is defined as that quantity of enzyme that will liberate o-nitrophenol at a rate of 1 mol min under the conditions of the assay. Calculate the activity (lactase activity per gram) of the enzyme preparation taken for analysis as follows: ALU/g = [(A S B)(25)]/[(ε)(15)(W)] in which A S is the average of absorbance readings for the Test Preparation; B is the average of absorbance readings for the blank; 25 is the final volume, in milliliters, of the diluted incubation mixture; is the mean absorptivity of the o-Nitrophenol Standards per micromole, 15 is the incubation time, in minutes, and W is the weight, in grams, of original enzyme preparation contained in the 0.5-mL aliquot of Test Preparation used in the incubation.