Quicking Ractopamine Rapid Test isa competitive immunoassay for the semi-quantitative detection of the presence of Ractopamine residue in animal’s tissue.
Cut-off: 2,3 ,5ppb(The cut-off is flexible.Please contact us for details.)
Assay Time: 5 -10 min
Sample: Tissue (If you need to detect other samples as urine,feed or serum,please contact us for details.)
2. PRINCIPLE OF THE ASSAY
Quicking RactopamineRapid Test is based on competitive lateral flow immunochromatographic assay. The RAC-conjugate in the test zone will capture the immuno-gold (colloid gold-RAC antibody conjugate), when there is very little dissociative RAC in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if RAC is present in concentration of 3 ppb and above which explains a positive result.
3. TEST PROCEDURE
- Warm test device to room temperature.
- Collect tissue fluid from lean meat by one of the following ways.
a) Put minced or sliced meat into a properly sized plastic bag and freeze it. Thaw it 12 hours later. Cut a small hole in the bottom of the bag and collect the tissue fluid then.
b) Warm minced meat in a hot bath not more than 60°C and collect the tissue fluid.
c) For warm meat, collect the blood.
d) Squeeze the tissue fluid out of minced meat with a gauze.
- Stand it for a while and collect the supernatant as testing sample.
- Remove the cassette from foil pouch and place it horizontally on a flat surface.
- Transfer 3 drop of sample into the sample well“S”.
- Interpret the result in 5-10 minutes.
- A result read after 15 minutes is considered invalid.
4. INTERPRETATION OF RESULTS
Positive: Only clear C band appears.
Positive shows that the concentration of Ractopamine is at or above 3 ppb in the sample.
Negative: The presence of both C band and Tband.
Invalid: No coloured band appears in C zone regardless of whether T band appears.
The results are negative when the test is applied to detecting 100 ppm of Clenbuterol, Salbutamol, Trenbolone-acetate, Chloramphenicol, Aminoglycosides, Beta-Lactams, Macrolides,Quinolones, and Tetracyclines.
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foilpouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.