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A 10x microscope objective lens is considered a medium power objective lens. When used with a 10x eyepiece lens, it yields a total magnification of 100 times. A 10x lens focuses on details to enable scientists or hobbyists to view small specimens or samples under the microscope.
This kind of lens can also be utilized for low power, high power, and oil immersion. This is because this lens has a numerical aperture of 0.25, which is ideal for routine tasks. It offers decent resolving power.
However, a 10x objective lens is not used alone. In practical applications, it's paired with other lenses to increase magnification. People widely use it for microbiological research, cell culture, and material science.
Apart from this objective lens, users can also find objective lenses with different magnifications like 40x, 60x, or 100x.
10x objective lenses come in several types depending on the imaging application and specimen requirement. These are:
Achromatic Objective Lens
These are designed to limit chromatic and spherical aberrations. Users can easily identify them, as there are usually two colored rings. These lenses are ideal for general laboratory work and educational settings. This is because they provide significantly improved image quality over simpler lenses at a much lower cost.
Semi-Apochromats
These are manufactured to correct chromatic aberration better than achromatic lenses. Semi-apochromatic microscopes correct two chromatic aberrations and one spherical aberration. That means they offer better image quality than achromatic lenses. However, not as good as fully apochromatic lenses. Semi-apochromatic objective lenses are frequently applied in cellular biology and histology. After all, semis offer good color correction at a reasonable cost.
Apochromat Objective Lens
These are high-precision lenses designed to minimize chromatic and spherical aberrations. They have the best correction of abnormally chromatic lenses made of special glass types. These are most frequently applied in advanced imaging, where precision and specimen detail are critical. For instance, these objectives are vital in photomicrography of specimens for fine structural analysis.
Some of the important specifications concerning the 10x objective lenses include:
Numerical Aperture (NA)
The NA of the 10x objective lens is 0.25. It controls the amount of light entering the lens and affects the resolution. The higher the NA value, the better the lens resolves fine specimen details. In addition, NA also determines the lens's ability to function with varying types of immersion media.
Tube length
Tube length is the distance between the eyepiece and objective lenses manten area. In standard microscopes, this distance is frequently 160 mm. This distance is set to ensure that when used alongside compatible eyepieces, the objectives produce images in sharp focus.
Working distance
Generally, the working distance of a 10x objective lens is approximately 2-3 mm. This distance ensures that the lens can focus on the specimen without crushing or damaging it. Manufacturers commonly feature this lens in many kinds of microscopes, including biological and clinical ones.
Users should note that although its magnification power is relatively low compared to others, the 10x lens still provides a clear, focused view of fine details. This makes it perfect for routine microscopic examinations of various specimens.
Tuning Microscope
First, users should rotate the focus knob until the specimen comes into view and focused. Microscopy usually focuses on the region of interest, which is why precise focusing is vital. Using a lower power initial objective to find the area of interest, then switching to 10x, will make this easier.
Focusing
After getting a preliminary focus with a lower objective lens, users should switch it to 10x. They should make small adjustments using the fine focus knob to attain a sharp image. It is important to avoid crashing the lens into the slide because of the minimal working distance.
Zoom
Although 10x is not high compared to other objective lenses, it still provides adequate magnification for most routine applications. This makes it suitable for viewing larger cells, tissues, or microorganisms. Its relatively low power also offers a wider field of view, which is beneficial for scanning samples quickly.
Specimen Observations
This lens enhances details and the overall structure of specimens like bacteria, yeast, or human blood cells. Although it cannot view the finest details or structures, its ease of use makes it a go-to for routine examinations.
This lens is designed for high-power magnification of specimens, usually yielding a magnification of 400x when paired with a 10x eyepiece. This lens offers a more precise view of the specimen's surface structures, finer details, and intricate patterns. This makes it essential for users in pathology, material science, and microbiology.
Apart from this microscope lens, users can also find other objective lenses ranging from 4x to 100x.
Just like the 10x model, the 40x objective lens also has different types. These are:
Achromatic 40x Objective Lens
These are designed to reduce chromatic aberration. This helps in minimizing spherical aberration. Usually, the 40x achromatic objective lens has red and blue bands. These lenses provide improved image quality. This is especially when compared to simpler lenses.
Semi-Apochromats
These lenses offer better correction for chromatic and spherical aberrations than achromatic lenses. This makes them ideal for users who require good color fidelity and sharp images. Semi-apochromat lenses are an excellent balance between cost and performance. This is because they provide enhanced precision without the hefty price of fully apochromat lenses.
Stereomicroscope Lens
These are specially designed to provide a three-dimensional view of the specimen. As a result, users see the surface details and texture. This makes them ideal for industrial inspection and biological studies.
Apochromat Objective Lens
These are constructed to correct spherical and chromatic aberrations. They provide the sharpest and most accurate images among all objective types. Although these are expensive, businesses should consider buying them. After all, they are indispensable in advanced microscopy applications, especially in photomicrography.
Important specifications for the 40x objective lens include:
Numerical aperture
Usually, the NA for this objective lens is 0.65. This value enhances its light-gathering capacity. It also increases resolution compared to 10x.
Working distance
The working distance for this lens is around 0.5 mm. This minimizes the distance between the lens and the specimen compared to 10x. This requires careful focusing to avoid crashing into the slide.
Tube length
Typical tube lengths for 40x objective lenses are 160 mm. This standardization ensures compatibility with most microscope models. This includes biological and clinical microscopes.
Tuning Microscope
Users need to ensure that the microscope is properly set up before switching on the objective lens. For precise focusing, it is advisable to find the area of interest using a lower power objective. This is because the 40x lens has a small field of view. So, locating the area of interest using a lower magnification is essential.
Focusing
Once they have located the area, they can make minor adjustments using the fine focus knob. This is where the tenuous balancing act comes in. Because of the short working distance, users must be extra careful with their focusing. Just a small amount of excessive pressure will end up crashing the lens into the slide.
Specimen Viewing
This objective lens strikes the right balance between moderate magnification and good detail. This makes it suitable for viewing larger cells, tissues, or microorganisms. Although it's not the most powerful, the 40x lens is ideal for routine examinations.
Zooming
This microscope lens is specially designed to provide a clear view of large cells, tissues, and microorganisms. Even though it cannot view ultra-fine details, it is still simple to use. This makes it ideal for routine checks.
This is a high-power microscope objective lens. It normally provides detailed magnification of specimens, usually around 600x when combined with a 10x eyepiece. This lens is ideal for users requiring sharp and clear views of thin sections, small cells, or intricate materials. People often use it in cellular biology, histology, and material science.
Like other objective lens types, this one also has a variety. They include:
Fluorite 60x Objective
Also referred to as the ‘semi-apochromat’ lens, this applies to the 60x objective lens made with fluorite or calcium sodium glass. It corrects chromatic aberrations better than the 40x objective lens. This provides sharp, bright images. Fluorite lenses are frequently featured in applications needing fine detail and color fidelity.
Phase Contrast
These 60x lenses are specifically manufactured to enhance contrast in unstained biological specimens. They have special rings or plates that alter the path of light. This makes them ideal for viewing living cells or transparent microorganisms.
Stereomicroscope Lens
These are specially designed to provide a three-dimensional view of the specimen. As a result, users see the surface details and texture. Basically, these objective lenses enhance precision by offering depth perception. This advantage makes them critical for various applications. For instance, inspecting industrial components or studying biological specimens.
Achromatic Objective Lens
These are designed to reduce spherical and chromatic aberration. An achromatic fluorescence objective usually has two colored bands that help the user identify them. In addition, they correct for two wavelengths of light. This allows for improved image quality. These lenses are ideal for routine biological microscopy. After all, they balance cost and performance.
Here are some key specifications of the 60x lens:
Numerical aperture
Usually, the numerical aperture for this objective lens is 0.75. This value enhances its light-gathering capacity. It also increases resolution compared to the 10x objective lens.
Working distance
For this lens, the working distance is approximately 0.2 mm. This distance minimizes the gap between the lens and the specimen. Although this has the advantage of increased magnification power, it requires careful focusing to avoid contact with the slide.
Tube length
Typical tube lengths for this objective lenses are 160 mm. This standardization ensures compatibility with most microscope models. These include both clinical and biological microscopes.
Tuning Microscope
To focus on the specimen, start by locating an area using a lower objective lens. After identifying the area, they can switch to this high-power lens. It has a small field of view, so careful techniques for locating the area are crucial.
Focusing
Once they have the area in view, minor adjustments using the fine focus knob will bring the specimen into sharp focus. Users should avoid excessive pressure due to the small working distance. After all, just a small incorrect amount of pressure will end up crashing the lens into the slide.
Specimen Viewing
This objective lens provides detailed views of larger cells or subcellular structures. This makes it ideal for examining thin tissue samples in histology or intracellular components in cell biology.
Zooming
Although not the most powerful, the 60x lens is ideal for routine examinations. It strikes an excellent balance between good detail and ease of use. This makes it suitable for viewing cells, tissues, or complex materials.
In order to get the best objective lens, buyers need to consider some factors. Below are these factors:
Supplier Reliability
Just like any other product, buyers need to ensure that the supplier is reliable. They should provide such essential details as the kinds of materials used to manufacture the product, its specifications, and how to use it.
Minimum order quantity
Buyers should also ensure that the supplier's MOQ is in line with their business needs. They should also consider the scalability of the orders they place as their business grows.
Shipping & logistics
Buyers should also consider the shipping and logistics of the 10x microscope objective lens they buy. They should look for suppliers who offer the shipping mode that is most suitable for them. They also need to consider the shipping costs and delivery times. This is what will enable them to make an informed decision.
Product quality
Quality is one of the most crucial factors to consider. After all, it determines the kind of experience the customers will have. Buyers should look for 10x microscope objective lens designed using quality materials. They should also research the scientific hardware company’s reputation and read reviews to ensure any lens they settle for has quality manufacturing.
Price
Buyers will consider this factor alongside the quality of the product. They will shop and compare prices, all the while being cognizant of any additional costs like shipping and taxes. Only after weighing these costs against the quality and potential bulk purchase discounts will they make a decision.
A1. This objective lens is used for clear, detailed views of cellular structures, tissue preparations, and small organisms. It strikes an excellent balance between good field diameter and depth of field.
A2. It provides moderate magnification power, which is ideal for routine applications like bacteria observation. Others provide more detail or less detail, depending on their specifications.
A3. Yes, there are. Depending on the specific needs, other objectives like the 40x or 60x may serve in a pinch. However, none of them would be as effective when it comes to general-purpose microscopy as this one.
A4. They have a NA of about 0.25, a working distance of 2-3mm, and a tube length of 160 mm. These characteristics help make this lens suitable for many routine microscopy applications.
A5. The essential factors they consider include the lens's quality and type, the reputation of the supplier, and the costs involved.
